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KMID : 0525720130180020111
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Journal of Chitin and Chitosan
2013 Volume.18 No. 2 p.111 ~ p.116
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Effects of Deep-Sea Water on Metastasis Inhibition of Chitosan Oligosaccharide in Human Breast Cancer Cells
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Kwon Yun-Suk
Chun So-Young
Lee Do-Hyung
Nam Kyung-Soo
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Abstract
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Metastasis is a major cause leading to high mortality to breast cancer patients. Invasiveness of breast cancer cells is associated with matrix metalloproteinases (MMPs), urokinase type plasminogen activator (uPA), and its receptor (uPAR), and angiogenesis modulated by vascular endothelial growth factor (VEGF) is necessary for growth of cancer cells in distant site. In order to evaluate the effect of deep-sea water (DSW) on antimetastatic effect of chitosan oligosaccharide (COS-L, MW ≤ 1,000) in MCF- 7 human breast cancer cells, we estimated inhibitory effect of COS-L (0.1~5 mg/mL) dissolved in hardness 0 (H 0) DSW on TPAinduced MMP-9 expression. And then expression changes of TPA (phorbol-12-myristate-13-acetate)-induced MMP-9, uPA, uPAR, and VEGF by COS-L (3 mg/mL) in DSW with various hardness (200, 400, 800, 1,500) were also assessed. Expression of MMP-9 induced by TPA was diminished in a range of 3~5 mg/mL of COS-L. Moreover Interestingly, DSW of H 400~1,500 significantly enhanced inhibitory effect of COS-L on expressions of TPA-induced MMP-9, uPA, uPAR. In the case of VEGF, DSW of over H 800 had a significant inhibitory effect on the expression. The results of this study indicate that DSW promotes antimetastatic effect of COS-L in MCF-7 human breast cancer cells by decreasing expression of metastastic factors (MMP-9, uPA, uPAR) and angiogenic factor (VEGF).
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KEYWORD
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Deep-sea water, Chitosan oligosaccharide, Breast cancer, Matrix metalloproteinase-9, Urokinase type plasminogen activator
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